What does REF mean in BRITISH MEDICINE
Restriction Enzyme Fingerprinting (REF) is a method used to identify different types of bacteria or viruses. It works by analyzing the DNA sequence of a sample and looking for specific patterns that are unique to that particular organism. By doing this, REF can be used to accurately identify unknown organisms or determine how closely related two organisms may be. REF is commonly used in medical laboratories and forensic science settings to identify infectious agents quickly and accurately.
REF meaning in British Medicine in Medical
REF mostly used in an acronym British Medicine in Category Medical that means restriction endonuclease fingerprinting
Shorthand: REF,
Full Form: restriction endonuclease fingerprinting
For more information of "restriction endonuclease fingerprinting", see the section below.
Explanation
REF works by using endonucleases, which are enzymes that cut DNA at specific sites, called restriction sites. Each organism has a unique pattern of these restriction sites, making it possible for researchers to easily differentiate between different species. Restriction enzymes work on double-stranded DNA molecules, cutting them at restriction sites into smaller segments referred to as fragments. The size of the fragments depends on the restriction enzyme being used and the type of bacteria or virus that is being studied.
The next step in REF involves separating the fragments according to their size using gel electrophoresis. This technique allows scientists to run each fragment through an electric field and observe them under ultraviolet light where they will fluoresce due to their length. By comparing the number and sizes of the fragments from different samples, researchers can then identify particular kinds of bacteria or viruses with greater accuracy than other methods such as serological testing or PCR analysis.
Essential Questions and Answers on restriction endonuclease fingerprinting in "MEDICAL»BRITMEDICAL"
In summary, REF (Restriction Enzyme Fingerprinting) is a powerful tool for rapidly identifying unknown organisms or determining how closely related two organisms may be. It works by cutting DNA molecules into smaller segments at specific restriction sites with endonucleases before those fragments are separated based on their size using gel electrophoresis. This technique offers a more accurate way of identifying bacterial or viral infections than traditional methods such as serologic testing or PCR analysis and is widely used in medical laboratories and forensic science settings alike.
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