What does LCM mean in UNCLASSIFIED
Laser Capture Microdissection (LCM) is a molecular biology technique that allows for the precise isolation of specific cells or cell populations from a tissue sample. The process involves using a focused laser beam to cut and extract the desired cells, which can then be analyzed for various purposes, such as gene expression studies, DNA sequencing, or protein analysis.
LCM meaning in Unclassified in Miscellaneous
LCM mostly used in an acronym Unclassified in Category Miscellaneous that means Laser Capture Microdissection
Shorthand: LCM,
Full Form: Laser Capture Microdissection
For more information of "Laser Capture Microdissection", see the section below.
LCM: Laser Capture Microdissection
Introduction
Principle of LCM
LCM is based on the principle of laser-induced cell ablation. A typical LCM system consists of a laser, a microscope, and a computer-controlled stage. The tissue sample is mounted on a slide and visualized under the microscope. The operator uses a software interface to select the cells of interest. The laser is then focused onto the selected cells, and a short pulse of energy is delivered. This pulse vaporizes the cells, separating them from the surrounding tissue.
Applications of LCM
LCM has numerous applications in biomedical research, including:
Gene Expression Studies: LCM can isolate cell populations with specific gene expression patterns, allowing researchers to study gene regulation and differential gene expression between different cell types.
DNA Sequencing: LCM can obtain DNA from specific cells or cell populations, enabling targeted sequencing and identification of genetic variants or mutations associated with diseases.
Protein Analysis: LCM can collect proteins from specific cells or cell populations, allowing for proteomic analysis to study protein expression and post-translational modifications.
Pathology: LCM can isolate cells from diseased tissues for diagnostic purposes, such as identifying tumor cells or specific cell types involved in inflammatory processes.
Advantages of LCM
Precision: LCM allows for the isolation of specific cells or cell populations with high precision, minimizing contamination from surrounding tissue.
Specificity: The laser-based approach ensures that the isolated cells are representative of the targeted cell population, reducing biases introduced by manual cell selection methods.
Versatility: LCM can be applied to various tissue types and sample sizes, making it a versatile technique for different research applications.
Essential Questions and Answers on Laser Capture Microdissection in "MISCELLANEOUS»UNFILED"
Laser Capture Microdissection is a powerful technique that enables the precise isolation of specific cells or cell populations from a tissue sample. Its high precision, specificity, and versatility make LCM a valuable tool for various biomedical research applications, including gene expression studies, DNA sequencing, protein analysis, and pathology.
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