What does BHIS mean in UNCLASSIFIED

Brain heart infusion supplemented, or BHIS, is a culture media used to grow and maintain bacteria and other microorganisms in a lab. It's beneficial for studying the growth and behavior of different organisms. This media contains beef extract, peptone, sodium chloride, dextrose, and yeast extract to provide essential nutrients for the microbes.

You can purchase pre-made BHIS from a lab supply store or online seller. To make it yourself, you'll need to mix 4.5 g of brain heart infusion powder with 1 liter of distilled water. Once it's dissolved, autoclave at 121°C for at least 15 minutes to sterilize it before use.

With its rich nutrient mix, BHIS is an ideal medium for culturing viruses as well as gram-positive and gram-negative bacteria. Yeast cells in particular tend to grow very quickly in this media due to its components like peptone and dextrose.

Yes! This culture media has been designed specifically for lab use and is considered non-hazardous when proper safety measures are taken — such as wearing gloves and washing your hands afterwards — just like with any other laboratory experiment involving microbial cultures.

A typical concentration used in most experiments is 5-10%. That means 5-10 g/L of BHIS should be mixed with distilled water before autoclaving it for sterilization.

No additional nutrients are usually needed since BHIS contains all the necessary building blocks such as beef extract, peptone, sodium chloride etc., though you may want to supplement it with vitamins or other compounds depending on the kind of experiment being run and what type of organism is being studied.

Yes! To make blood agar plates using BHIS medium simply add 5-10% sheep’s blood after autoclaving the mixture then pour into sterile Petri dishes prior to use. The blood will provide an additional source of nutrients that will allow certain types of bacteria including some pathogenic ones to be grown on this media instead of just plain tryptic soy agar which cannot support their growth.