What does PRM mean in UNCLASSIFIED

Parallel Reaction Monitoring (PRM) is an analytical technique used to quantitatively measure the levels of peptides and proteins in complex biological samples. It utilizes multiplexed targeted liquid chromatography–mass spectrometry, allowing for the simultaneous detection of many target compounds in a single run.

PRM uses a combination of liquid chromatography (LC) and mass spectrometry (MS) to identify and quantify target peptides or proteins from complex biological samples. The sample is first separated according to its molecular weight by LC, before being ionized and detected by MS. This allows for the accurate identification and quantification of the target compounds.

PRM offers several advantages over traditional techniques for analyzing peptides and proteins. It has greater sensitivity than other methods, allowing for accurately detecting low concentrations of targets even in complex samples. Additionally, it can analyze multiple drugs at once, saving time compared to single-drug analysis methods. Finally, it eliminates any bias caused by manual sample preparation or interpretation.

PRM can be used to analyze a wide range of samples including serum, urine, cell lysates, tissue homogenates, as well as food and drug products. It can also be used to monitor components in reaction mixtures during drug synthesis processes or bioprocesses.

Yes, PRM has much higher sensitivity than other bulk analytical methods such as ELISA assays or Western blots. This makes it suitable for detecting drugs at very low concentrations, even in complex samples.

Generally speaking most triple-quadrupole LC-MS systems which include an Electron Ionization source will suffice, although more advanced systems such as Orbitrap instruments may be needed depending on the complexity of the sample matrix being analyzed.

As with all analytical techniques there are some limitations associated with using PRM such as matrix effects caused by compounds present in the sample that interfere with ionization efficiency or disrupt chromatographic separation; however these generally can be minimized through careful choice of solvent system and ionization conditions.

Yes, when combined with calibration curves constructed from known standards PRM can generate highly accurate quantitative results.

Depending on parameters such as LC gradient length and MS scan speed a single run using typical parameters will typically take between 15 minutes up to one hour or more.

To get the most out of your instrument you should receive proper training either through vendor provided courses or by utilizing knowledgeable staff experienced in bioanalytical method development.