What does BLCL mean in LABORATORY
BLCL stands for B Lymphoblastoid Cell Lines. BLCL is a type of cell line derived from human or mouse B-lymphocytes which are mutated by the Epstein-Barr virus. These cell lines are used to study the effects of drugs, viruses, and other agents on the immune system as well as for the detection and characterization of new antigens. BLCLs have also been used for vaccine development, in vitro selection experiments and gene editing. The advantages of using BLCLs include their wide availability, relatively low cost, ability to be frozen and thawed with no significant loss of viability, and known health status due to their origin from non-cancerous tissue.
BLCL meaning in Laboratory in Medical
BLCL mostly used in an acronym Laboratory in Category Medical that means B Lymphoblastoid Cell Lines
Shorthand: BLCL,
Full Form: B Lymphoblastoid Cell Lines
For more information of "B Lymphoblastoid Cell Lines", see the section below.
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Meaning
BLCLs are typically grown in suspension in a culture medium consisting of RPMI 1640 supplemented with fetal bovine serum (FBS). The cells are usually passaged every 3-4 days at 1:4 split ratio (1 volume of cells per 4 volumes of fresh medium) and should be monitored regularly to ensure a healthy growth rate and phenotype. The Epstein-Barr virus genetic information is encrypted into these cells’ DNA, causing them to produce different forms of viral proteins which make them useful for research into infectious diseases caused by this virus.
Usefulness
BLCLs are commonly used in immunology research as they offer a variety of advantages over primary B-lymphocytes such as better growth conditions, increased uniformity across samples and greater long term stability when stored cryopreserved. They can also be genetically modified more easily than primary B-lymphocytes due to their use of tetracycline gene induction systems or retroviral transduction protocols. BLCLs can also be used to test potential therapeutic compounds on the immune system or detect antigenic targets on tumor cells that may be relevant in cancer immunotherapy studies. Additionally, they can be used to characterize the generation of monoclonal antibodies or screen newly identified antigens for possible vaccine development purposes.
Essential Questions and Answers on B Lymphoblastoid Cell Lines in "MEDICAL»LABORATORY"
What are B-Lymphoblastoid Cell Lines?
B-Lymphoblastoid cell lines are established cell lines derived from a single B-lymphocyte. They have been engineered to a suspension culture and can be used in laboratory settings for biological research.
How can B-Lymphoblastoid Cell Lines be used?
B-Lymphoblastoid Cell Lines are used extensively in immunology, biochemistry, molecular biology, protein engineering and other fields of research. They have also been used in drug discovery as an important tool.
What are the applications of B-Lymphoblastoid Cell Lines?
B-Lymphoblastoid Cell Lines have many potential uses, including studying antibody production, gene expression regulation, signal transduction pathways, cell signaling networks, transcriptional programming and many other applications.
Are there any risks associated with using these cells?
As with any laboratory technique, there is some risk involved when working with any type of cell line. It is important to always follow good laboratory practices and take appropriate precautions when handling any type of biological sample.
What kind of information can be obtained from analyzing these cells?
By studying the expression patterns of genes within these cells it is possible to gain insight into the regulation of gene expression under various conditions. In addition, this type of analysis allows researchers to study the function of particular proteins within the cell or even identify new ones.
Is it possible to genetically engineer these cells?
Yes, genetic engineering techniques such as CRISPR/Cas9 can be used on the B-Lymphoblastoid Cell Line to manipulate genes by inserting or deleting specific pieces of DNA or RNA sequence into their genome.
How long can these cells cultures remain viable for experimentation?
Properly maintained cultured cells can remain viable for several weeks up to several months depending on their age and composition. It is important to monitor your cell lines regularly for signs of contamination or decline in viability.
Are there standard protocols that should be followed when working with these cells?
Yes, depending on what type of experiment you are conducting it is always best practice to refer to relevant guidelines and follow standard procedures when handling any type of cellular material Cultures should also be handled carefully as contamination is a major concern.
Final Words:
In summary, BLCL stands for B Lymphoblastoid Cell Lines which are somewhat unique cell lines derived from noncancerous human or mouse B-lymphocytes that contain EBV genetic information embedded within them. These cell lines offer various advantages over primary B-cells such as better growth conditions, increased uniformity across samples and greater long term stability when cryopreserved making them ideal for many immunology research applications including vaccine development studies, disease diagnosis assays and characterizing monoclonal antibody responses among others.