What does TBST mean in UNCLASSIFIED
TBS-T is an abbreviation for Tris Buffered Saline with Tween. It is a common solution used in the laboratory, particularly in cell culture, to rinse and wash cells prior to experimentation or storage. This solution can also be used as a buffer, a transport medium, and in various other biochemical experiments.
TBST meaning in Unclassified in Miscellaneous
TBST mostly used in an acronym Unclassified in Category Miscellaneous that means Tris Buffered Saline with Tween
Shorthand: TBST,
Full Form: Tris Buffered Saline with Tween
For more information of "Tris Buffered Saline with Tween", see the section below.
Essential Questions and Answers on Tris Buffered Saline with Tween in "MISCELLANEOUS»UNFILED"
What is Tris Buffered Saline with Tween?
Tris Buffered Saline with Tween (TBS-T) is a common solution used in the laboratory to rinse and wash cells prior to experimentation or storage. It can also be used as a buffer, a transport medium, and in various other biochemical experiments.
What does TBS-T do?
TBS-T helps protect cells from damage due to sudden changes in pH or osmolarity during experiments or storage. It is also useful for dissolving molecules that may otherwise not dissolve in water alone.
How do you prepare TBS-T?
To prepare TBS-T, use 10X Tris Buffer (pH 7.6), 0.01% Tween 20 and sterilized saline at 1X concentration. Adjust the pH of the solution if necessary before use.
What type of experiments use TBS-T?
TBS-T has many uses in laboratory experiments including cell washing and rinsing, preparation of tissues for imaging or analysis, protein extraction from bacterial cultures, DNA purification protocols and more.
Is there an alternative to using TBS-T?
Yes, another popular solution for lab use is phosphate buffered saline (PBS). PBS does not contain any detergents such as tween 20 like that found in tbs-t but still serves as an effective buffer and transporter medium when studying biological samples. However it should be noted that PBS may not always provide optimal results depending on the experiment being conducted so careful consideration should be given when selecting which buffer to use.
Final Words:
In summary, Tris Buffered Saline with Tween (TBS-T) is an important tool used within labs for cell washing/rinsing and various other applications involving biochemical experiments or buffers/transport media needed for different types of studies. While PBS can serve as an alternative option, it may not always yield ideal results depending on the experiment so careful consideration should be given when deciding which buffer to employ.