What does SPR mean in BRITISH MEDICINE
Solid phase radioimmunoassay, or SPR, is a laboratory technique used to measure the amounts of antigen in a sample. It is a powerful diagnostic tool in medical research and technology that researchers use to identify and quantify antibodies and other biological molecules with very high sensitivity. SPR relies on the specific binding of an antigen to its corresponding antibody. When using this method, researchers can measure less than one picomole (10−12 moles) of an antibody in a sample.
SPR meaning in British Medicine in Medical
SPR mostly used in an acronym British Medicine in Category Medical that means solid phase radioimmunoassay
Shorthand: SPR,
Full Form: solid phase radioimmunoassay
For more information of "solid phase radioimmunoassay", see the section below.
Definition
In simple terms, SPR is an analytic technology used for the determination of the chemical properties of biological samples. This test involves attaching a target antigen molecule onto a solid surface such as glass or plastic. The surface is then exposed to labeled antibodies which will bind to the antigen if present in the sample. Once bound, radioactivity from the labeled antibody can be measured and used to determine how much antigen is present in the sample.
Benefits
This technique offers many advantages over traditional methods for measuring antigens in fluids such as blood or urine. For example, it is more sensitive than typical enzyme-linked immunosorbent assays (ELISA), producing results that are both accurate and precise with greater sensitivity than ELISA variables such as absorbance readings or radioactive labels usually require additional equipment for ELISA assays while SPR does not need any extra hardware beyond its setup apparatus; this helps reduce costs significantly compared to ELISAs which often require additional equipment and/or expensive materials such as monoclonal antibodies synthesized specifically for certain tests. Additionally, by requiring fewer steps than most ELISAs (generally just up to four steps instead of six or more), SPR has been successful at reducing time needed for assay completion compared with other testing protocols associated with ELISAs.
Essential Questions and Answers on solid phase radioimmunoassay in "MEDICAL»BRITMEDICAL"
What is Solid Phase Radioimmunoassay (SPR)?
Solid Phase Radioimmunoassay (SPR) is a type of immunometric assay used to measure the concentration or activity of antigens or antibodies in biological fluid samples. It is commonly used in medical applications such as the diagnosis of infectious diseases, the determination of levels of hormones and therapeutic drugs in body fluids, and environmental monitoring.
How Does SPR Work?
In SPR, radioisotopes are used to label either an antigen or antibody, which are then mixed with the sample and solid support materials to form complexes. The complexes are then detected using a gamma counter. The amount of radioactivity present in each complex can be measured and used to determine the concentration or activity of the antigen or antibody present in the sample.
What Are The Advantages Of Using SPR?
Some advantages of using SPR over other immunometric assays include its greater sensitivity and specificity, faster results, increased reliability and reproducibility, improved accuracy in measuring small amounts of antigens or antibodies, lower cost compared to other assays, and lack of need for centrifugation prior to analysis.
What Is Measured With SPR?
Solid-phase radioimmunoassay can be used to measure both antigens and antibodies in various biological samples such as serum, plasma, cerebrospinal fluid (CSF), saliva and urine.
What Types Of Samples Can Be Used For SPR Analysis?
Most any type of biological sample can be used for solid phase radioimmunoassay analysis including serum, plasma, cerebrospinal fluid (CSF), saliva and urine.
Why Is Quality Control Important When Conducting an SPR Analysis?
Quality control measures must be taken when conducting an SPR analysis since a slight deviation from standards could lead to false results. Quality control helps ensure that all test results are accurate and reliable.
What Type Of Equipment Is Used For An SPR Studies?
To conduct an SPR study several pieces equipments may be required depending on specific requirements such as gamma counters and scintillation counters for measuring radioactivity; pumps for adjusting flow rates; solid supports such as plastic tubes/columns; magnetic stirrers for homogenizing samples; thermometers for controlling temperature during studies; mixing devices such as vortexers; syringes; spectrophotometers for measuring absorbance; pH meters for deciding acidity/alkalinity levels; centrifuges for separating components from solutions etc..
Final Words:
In summary, solid phase radioimmunoassay (SPR) provides researchers with an effective method for quantifying antigens present within biological samples quickly and accurately without the need for complicated equipment or reagents found in other assay techniques like ELISA. It offers excellent sensitivity compared with more traditional methods allowing smaller quantities of target molecules to be detected readily making it invaluable in diagnostics research and technology today.
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