What does SFM mean in UNCLASSIFIED
Serum free medium (SFM) is a type of culture medium used for cell lines and cultures which do not require the presence of animal serum in order to grow. It is commonly used in cell-culture research, particularly when culturing suspension cells and primary explants. SFM has many advantages over traditional media, including increased reproducibility, improved genetic stability, and easier manipulation of growth factors.
SFM meaning in Unclassified in Miscellaneous
SFM mostly used in an acronym Unclassified in Category Miscellaneous that means serum free medium
Shorthand: SFM,
Full Form: serum free medium
For more information of "serum free medium", see the section below.
Definition
SFM is an acronym for serum free medium; a type of cell culture that does not require the presence of animal or human serum in order to grow. SFM is typically composed only of essential elements necessary for cell growth and metabolic activity such as carbohydrates, amino acids, vitamins, minerals, salts and buffers. Other components such as hormones or growth factors can also be included in the formulation if needed.
Advantages
One advantage of serum-free culture media is that the use of animal-derived components can be avoided completely, resulting in lower cost media formulations with fewer environmental concerns related to sourcing materials from animals or exposure to pathogens present within serum. Additionally, since it does not contain any undefined components found within serum which may vary between batches or species sources, SFMs tend to offer more consistent performance characteristics than traditional media formulations. This increased consistency results in more predictable growth patterns from one culturing process to another.
Essential Questions and Answers on serum free medium in "MISCELLANEOUS»UNFILED"
What is Serum Free Medium (SFM)?
SFM is a type of cell culture medium that does not contain any bovine or other animal-derived serum. It is a nutrient-rich medium that contains all the essential synthetic components for cell growth and development, such as vitamins, hormones, and salts.
What are the benefits of using SFM?
SFM eliminates potential contaminants from animal serum and provides optimal growth performance for cells in vitro. Additionally, it reduces health risks associated with animal or human-derived sera by eliminating the threat of contamination.
Is SFM a complete medium?
Yes, SFM is usually supplemented with essential additional components such as essential amino acid and vitamins to ensure complete nutrients are available to support healthy cell growth and development.
Does SFM work with all cell types?
SFM can be used to grow a variety of different cells types; however, the optimal conditions for different cells may vary according to their specific requirements. Therefore, some modification of the basal composition might be necessary before using it to cultivate certain cell lines.
How can I prepare my own media using SFM?
Preparation instructions vary depending on the type of media you want to use; however, there are some general steps that you should follow when making SFM. First, add all required components – such as carbohydrates, vitamins and trace elements – into an appropriate container and mix them together thoroughly. Then adjust pH levels if needed before adding antibiotics for microbial protection if desired. Finally, autoclave or filter sterilize before use if necessary.
How do I store my prepared SFM?
Prepared SFMs should be stored at 4°C in airtight containers away from light sources in order to maintain sterility and keep its quality intact over time. If not used immediately after preparation, sulfide containing compounds present in some amino acids may oxidize resulting in poor growth performance or even death of cultured cells when added to media.
Can I add antibiotics into my prepared SFM?
Adding antibiotics such as penicillin or streptomycin into your prepared media can help protect against microbial contamination during culturing of cells; however, you should always confirm compatibility with your target cell line before doing so.
What is the shelf life of pre-made Serum Free Mediums (SFMs)?
The shelf life of pre-made Serum Free Mediums (SFMs) is typically 12 months when stored under recommended conditions - suh as at 4°C away from direct light sources - providing those appropriate procedures were followed during production and packaging.
Final Words:
Due to its reliable performance characteristics and decreased variability relative to traditional cell culture media formulations containing animal serums, SFM has become increasingly popular among researchers wishing to take advantage of its well-defined properties in their workflows. In addition to these benefits associated with obtaining higher yields and levels of reproducibility by using SFMs compared with other types of media formulations using animal serums, the overall cost savings associated with using this type of media make it a cost-effective alternative for many laboratories.
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