What does SBH mean in HUMAN GENOME

SBH is short for Sequencing by Hybridization, which is a method of DNA sequencing that uses hybridization to determine the order of nucleotides in a molecule.

In SBH, a sample containing unknown sequences is mixed with known probes that have complementary sequences. The probes bind to their complementary strands on the sample's DNA molecules, which can then be read off in sequence from the probe binding site.

One advantage of using SBH over other methods is its ability to detect polymorphisms or mutations present in the sample. Also, it is relatively cost effective and can sequence entire genomes efficiently.

Some major drawbacks include the increased risk of data errors due to small differences between probes and target sequences, as well as problems with low-quality samples leading to inaccurate results.

It's important to note that while this method has been used successfully on various species including humans, it may not be suitable for certain applications due to its lack of scalability and sensitivity compared to other methods such as next-generation DNA sequencing platforms.