What does RPLC mean in UNCLASSIFIED

Reverse Phase Liquid Chromatography (RPLC) is a type of chromatographic method used to separate and purify compounds. It utilizes two phases; one stationary containing an adsorbent and one mobile containing a solvent.

In RPLC, the compound being analyzed is dissolved in the mobile phase and applied to the column. Then, through the process of partitioning, the sample binds to the stationary phase where it is then separated from other components by elution with various solvents having different polarities.

RPLC can be used in many industries including biochemistry, pharmaceuticals, environmental sciences, forensics, drug testing and more. Its ability to separate complex mixtures makes it particularly useful for research applications such as protein purification and analysis of complex biological samples.

Solvents with different polarities can be used depending on what needs to be separated from the sample mixture. Commonly used solvents include acetonitrile, water or methanol to elute non-polar molecules; and buffers like phosphate or tris buffer to elute polar molecules with different affinities towards the stationary phase.

Other chromatographic methods such as normal-phase liquid chromatography (NPLP) or size exclusion chromatography (SEC) also utilize two phases to separate compounds but differ in their mechanism of separation based on polarity and molecular weight of analytes respectively.