What does RPLC mean in UNCLASSIFIED

Reversed Phase Liquid Chromatography (RPLC) is a chromatographic technique used to separate and analyze compounds in a solution. It involves the interaction of the non-polar mobile phase with the more polar stationary phase to move solutes through the column.

RPLC can be used to analyze organic molecules, proteins, peptides, carbohydrates, drugs, metabolites, and other biological or pharmaceutical compounds.

RPLC is able to provide high resolution separations in shorter times than other techniques, reducing analysis time and costs for applications that require analysis at speed. Additionally, RPLC offers good sensitivity and reproducibility due to its high resolving power.

A HPLC system consists of an integrated pump that moves fluid through a flow cell connected to an analytical column containing stationary phases. The mobile phases are then introduced into the tablet via an injector port before being collected by detectors such as UV-Vis spectrophotometer or fluorescence detector. [END]

Q5: How are separations monitored during RPLC?

A5: Separations are typically monitored by means of UV absorbance or refractive index detector during normal reversed-phase chromatography with hydrophobic analytes present in solution matrix having little influence on elution profile monitoring method employed for qualitative analysis or quantitative methods like peak area integration for concentration determination purposes.