What does RGQ mean in PRODUCTS

Rotor-Gene Q is a thermal cycler, or PCR machine, that offers real-time quantitative PCR capability. It features a 48-well block with an independent temperature control for each well. This enables granular control of both sample temperatures and the transfer of heat to the sample, producing faster and more accurate results.

The Rotor-Gene Q utilizes a process called polymerase chain reaction (PCR) in order to amplify and detect nucleic acids such as DNA and RNA from samples. The sample is first heated to dissolve the DNA and then cooled to prime the polymerase enzyme, which then adds ‘building blocks’ or additional copies of the target sequence. The reaction cycles between heating and cooling several times (typically dozens) while simultaneously recording fluorescence generated by fluorescent probes in each well that indicate the presence of any target sequences present in the sample.

Rotor-Gene Q can be used for various applications such as gene expression profiling, SNP genotyping, copy number variation detection, RNA quantification, multiplex assays, miRNA quantification and sequencing library preparation.

There are two types of data analysis methods available on the Rotor-Gene Q – qualitative analysis which focuses on distinguishing between different amplicons using melting curve analysis; and quantitative analysis which determines exact concentrations of target amplicons within samples using Comparative Ct Method (CqM).

Yes, users can access preloaded protocols found in Roche’s Applied Science Library for easy set up without programming steps for common qPCR experiments. Additionally users may create their own customized experiments using professional software that is included with your purchase

Yes hardware upgrades are available enabling additional capabilities including gradient capability and larger block formats via interchangeable blocks or upgrade kits among other things. Please consult your user manual for specific instructions regarding these options