What does RACE mean in HUMAN GENOME

RACE stands for Rapid Amplification of cDNA Ends. It is a molecular biology technique used to identify and clone the ends of mRNA transcripts.

The RACE technique enables researchers to explore the ends of genes, which can provide insights into gene function and expression. Additionally, it allows researchers to clone full-length cDNAs that are difficult or impossible to obtain by traditional methods.

In order to use the RACE technique, researchers must create a double-stranded cDNA library from RNA isolated from cells or tissues. Next, an end-specific primer is used in conjunction with PCR during an initial round of amplification. Amplified products are then cloned into a vector for sequencing and subsequent rounds of PCR amplify larger fragments from any sequences present in the original cDNA library.

Any sample obtained from cells or tissues that contain mRNA molecules can be analyzed using the RACE technique. This includes total cellular RNA or specific types such as messenger RNA (mRNA), ribosomal RNA (rRNA), transfer RNA (tRNA) etc.

Different technologies that may be utilized in conjunction with the RACE technique include Reverse Transcription Polymerase Chain Reaction (RT-PCR) and cloning techniques such as ligation or recombinant DNA technology. Additionally, sequencing techniques such as Sanger sequencing can also be employed if needed.