What does PMME mean in UNCLASSIFIED

Primary mouse mammary epithelial (PMME) cells are a type of cell that originates from the mammary glands of mice. They can either be isolated directly in the laboratory or cultured using appropriate methods. PMMEs have been used extensively to study processes related to the development, differentiation and function of this gland in vitro.

PMMEs are typically obtained from mammary glands by enzymatic dispersion technique after euthanizing the mice. The extracted cells can then be incubated for several hours with an enzyme mixture that will break down cell-cell contact and allow for single-cell isolation.

PMMEs can be used for a variety of research purposes, including studying tissue-specific gene expression, analyzing cell cycle progression and behavior during differentiation and remodeling, as well as exploring developmental signaling pathways involved in organ formation and maintenance.

PMMEs should be stored in liquid nitrogen at temperatures between -196°C and -150°C to ensure their long-term viability prior to use. For short-term storage, they can also be kept on ice or at 4°C with culture media containing 10% fetal bovine serum or other suitable nutrient additives such as growth factors, hormones or antibiotics.

When properly stored at liquid nitrogen temperatures (-196°C and -150°C), PMMEs can remain viable for extended periods of time—often up to 24 months or more. However, once thawed from cryogenic temperatures, their overall viability decreases significantly with time depending on the environment they're kept in.

Depending on what specific task you're using the PMMEs for, there is specialized equipment required such as centrifuges for cell pellet preparation, pipettes for precise reagent addition, microscope slides for observing morphological changes over time, etc.

Yes! Due to their close relationship with mice tissues and organs it's important to observe high hygiene standards when handling these types of samples; disposable gloves should always be used when manipulating them directly and appropriate disinfectants applied regularly as needed.

Although using these types of well-established cell lines is an option available to researchers looking into studying concepts related to the development of the mammalian breast tissue in vitro, they may not provide accurate results due solely based on species differences alone. With that being said though; depending on your research objectives it may still present a valid choice depending on what data you are looking obtain specifically.