What does MMFT mean in LABORATORY
The Modified Microcomplement Fixation Test (MMFT) is a diagnostic test for the detection of certain autoimmune diseases, such as systemic lupus erythematosus (SLE), systemic sclerosis (SSc), and antiphospholipid syndrome (APS). It is used to measure the presence of autoantibodies in the patient's plasma. Autoantibodies are antibodies produced by the body that attack its own cells, leading to an auto-immune disorder. The MMFT is a medical test that measures a protein fraction, known as C3c, that has been found to be elevated in these conditions. In this test, special reagents are used to detect different fractions of C3c within the plasma of a patient and then analyze results.
MMFT meaning in Laboratory in Medical
MMFT mostly used in an acronym Laboratory in Category Medical that means Modified Microcomplement Fixation Test
Shorthand: MMFT,
Full Form: Modified Microcomplement Fixation Test
For more information of "Modified Microcomplement Fixation Test", see the section below.
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What is MMFT?
The Modified Microcomplement Fixation Test (MMFT) measures levels of C3c - a fragment of one particular complement factor - in order to diagnose several autoimmune disorders. Complement factors are proteins produced by the body which help it recognize foreign substances and initiate an immune response. When there’s an overactive immune system with too many autoantibodies present, C3c will be present at higher than normal amounts; thus it serves as a marker for autoimmune diseases. To measure these levels accurately and reliably, specific reagents are utilized so that only C3c levels can be measured from within the plasma sample.
How is MMFT Applied?
The application of Modified Microcomplement Fixation Test requires collection of a plasma sample from the patient being tested and handling with special reagents which bind specifically with C3c fragments. To ensure accuracy and reliability, comparison samples may also be taken from healthy subjects for comparison against those taken from patients suspected to have an autoimmune disease through analysis of immunoglobulin classes such as IgG or IgM on ELISA readers or other methods depending on availability. These samples measure not just general levels but also subclasses such as IgG1 or IgG4.
Essential Questions and Answers on Modified Microcomplement Fixation Test in "MEDICAL»LABORATORY"
What is the Modified Microcomplement Fixation Test?
The Modified Microcomplement Fixation Test (MMFT) is an immunological technique that measures the antigen-antibody reaction between a given antigen and its corresponding antibody or antisera. This test is used to measure the amount of antigen present in a sample, as well as the degree of binding between an antigen and its associated antibody.
How does the MMFT work?
The MMFT works by exposing an antigen sample to a complement protein known as C3b, which acts like a glue to bind together an antibody and its corresponding antigen. This causes the formation of "micro-complexes" which can be detected using specific antibodies labeled with fluorescent molecules. By quantifying these measured complexes, the amount and efficacy of both antigens and antibodies can be determined.
Is there any difference between MMFT and other similar tests?
Yes, there are several notable differences between MMFT and other tests such as ELISA or Western blotting. Firstly, MMFT uses significantly lower quantities of either antigens or antibodies than other testing procedures. Additionally, results obtained through this method are generally more accurate since smaller micro-complexes are measured instead of just single ones. Lastly, this technique requires less time for completion due to its automation process.
What kind of samples can be tested with MMFT?
The Modified Microcomplement Fixation Test has been found to be applicable for all types of liquid samples including serum, plasma, ascites fluid or saliva. Samples containing proteins like those found in recombinant DNA products can also be tested using this method.
Can I use my own reagents to perform MMFT?
While it is possible to use one's own reagents in performing this technique, we recommend that you obtain pre-made reagents from commercial suppliers for improved accuracy and reliability. Moreover, some specialised reagents that are required for optimal performance may not be readily available on your end.
How long does it take for results to become available after performing an MMFT test?
Normally it takes 1-2 days for results becoming available following the completion of an MMFT test depending on the protocol employed and number of samples being evaluated at once. Nonetheless, it should be noted that certain factors such as environmental conditions can influence testing time.
Final Words:
The Modified Microcomplement Fixation Test (MMFT) is used in diagnosing various auto-immune disorders by measuring elevated levels of C3C present in the plasma sample obtained from patients suspected to have auto-immune disease like SLE or APS etc.. Special reagents are specifically employed so that only C3C fragments can be detected accurately and reliably along with comparison samples taken from healthy individuals for clarification purposes when necessary. This method helps physicians correctly identify auto-immune disorders before resorting to more expensive treatments and/or therapies.
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