What does HTFA mean in UNCLASSIFIED

HTFA is High Throughput Fluorescence In Situ Hybridization (FISH) Analysis. It is a powerful method used to detect and quantify the presence or absence of RNA or DNA molecules in a sample.

The primary advantage of using HTFA is its ability to quickly and accurately analyze large amounts of data. It also reduces cost associated with molecular biology experiments by reducing the need for labor intensive assay optimization. Additionally, it provides greater sensitivity than other methods, allowing for more precise quantification of small amounts of target molecules.

HTFA works by utilizing fluorescent dyes that bind specifically to targets such as mRNA or DNA strands. These dyes emit light when exposed to light sources such as lasers which can then be detected by specialized detectors resulting in an image which can be analyzed computationally or manually.

Any sample containing nucleic acids such as cell culture samples, tissue sections and single cell suspensions can be analyzed with HTFA. Additionally, samples containing complex mixtures such as environmental samples or clinical specimens can also be analyzed.

The amount and relative abundance of specific target sequences, genetic variations between different samples, mutations within one sample, gene expression levels across different conditions and much more can all be obtained from HTFA analyses.