What does CCPCR mean in CHEMISTRY
CCPCR stands for chemical cycling polymerase chain reaction. It is a variation of traditional PCR that uses reagents which are consumed, consequently generating the desired product without the need for post-reaction purification. CCPCR increases sensitivity and reduces the amount of required reagents in complex multiplex PCR processes.
CCPCR meaning in Chemistry in Academic & Science
CCPCR mostly used in an acronym Chemistry in Category Academic & Science that means chemical cycling polymerase chain reaction
Shorthand: CCPCR,
Full Form: chemical cycling polymerase chain reaction
For more information of "chemical cycling polymerase chain reaction", see the section below.
Essential Questions and Answers on chemical cycling polymerase chain reaction in "SCIENCE»CHEMISTRY"
What is CCPCR?
CCPCR stands for chemical cycling polymerase chain reaction. It is a variation of traditional PCR that uses reagents which are consumed, consequently generating the desired product without the need for post-reaction purification.
Why use CCPCR instead of regular PCR?
CCPCR increases sensitivity and reduces the amount of required reagents in complex multiplex PCR processes. Additionally, it does not require post-reaction purification and can be used with lower amounts of sample material.
How does CCPCR work?
In CCPCR, a mixture of DNA polymerase and four nucleotides (A, T, G, C) is mixed with template DNA and allowed to amplify, then heated to denature double stranded products into single strands followed by cooling to anneal primers on single strands. The cycle repeats until all four nucleotides interact with each other in order to form new copies of DNA and create larger amounts of amplified products.
What are some advantages of using CCPCR?
Advantages include increased accuracy, improved yield rate from fewer false positives due to mispriming and reduced time expenditure from avoiding purifying after amplification has occurred. Additionally, because no additional cleanup steps are necessary after amplification has been completed, it significantly reduces time consumption as well as cost savings in terms of purchasing expensive kits or columns used for clean up.
Are there any disadvantages to using CCPCR?
A disadvantage could be the necessity for uniform temperatures due to certain components not being resistant to rapid temperature changes during thermocycling.
Final Words:
In summary, Chemically Cycling Polymerase Chain Reaction (CCPCR) is an advanced version of normal PCR that offers increased accuracy and yields at a lower cost compared to traditional PCR techniques requiring post-amplification clean up steps such as column preparation or kit purchases. Its utilization can be beneficial in multiplex analysis processes where low levels or small quantities samples require highly sensitive analysis results promptly achieved at a reasonable cost-effectiveness level.