What does TCM mean in CULTURE

TCM is a specialized nutrient-rich liquid that provides optimal conditions for the growth and maintenance of plant tissues or cells in a laboratory setting. It typically contains essential nutrients, growth regulators, and other supplements necessary for the survival and development of the plant material.

There are various types of TCMs, each formulated to meet the specific needs of different plant species or tissues. Some common types include Murashige and Skoog (MS) medium, Gamborg's B5 medium, White's medium, and Woody Plant Medium (WPM).

TCM serves as the nutritional backbone for plant tissues grown in vitro (outside the plant). It provides essential nutrients, hormones, and other factors that facilitate cell division, differentiation, and growth. TCM also helps maintain the physiological and metabolic stability of the cultured tissues.

TCM is typically prepared by dissolving pre-mixed powder formulations or individual chemicals in sterile water. The pH of the medium is adjusted to the desired range, and the solution is sterilized to eliminate any contaminants. The final medium is stored under sterile conditions until use.

Common ingredients found in TCM include macronutrients (e.g., nitrogen, phosphorus, potassium), micronutrients (e.g., iron, manganese, zinc), vitamins, amino acids, sugars (e.g., sucrose, glucose), growth regulators (e.g., auxins, cytokinins), and anti-oxidants.

Growth regulators are hormones or other substances that influence the growth and development of plant tissues. In TCM, they play a crucial role in regulating cell division, differentiation, and organogenesis. Auxins promote root formation, while cytokinins stimulate shoot development.