What does BGBB mean in UNCLASSIFIED
BGBB stands for Brilliant Green Bile Broth. It is a selective and differential medium used in microbiology to isolate and differentiate Salmonella and Shigella species from other bacteria.
BGBB meaning in Unclassified in Miscellaneous
BGBB mostly used in an acronym Unclassified in Category Miscellaneous that means Brilliant Green Bile Broth
Shorthand: BGBB,
Full Form: Brilliant Green Bile Broth
For more information of "Brilliant Green Bile Broth", see the section below.
BGBB Composition and Principle
BGBB contains peptone, meat extract, bile salts, lactose, sucrose, and brilliant green dye. The bile salts and brilliant green inhibit the growth of most Gram-positive bacteria and other enteric bacteria, while allowing Salmonella and Shigella to grow.
- Salmonella ferments lactose and sucrose, producing acid and gas, which turns the medium yellow and forms bubbles.
- Shigella does not ferment lactose or sucrose, but it produces hydrogen sulfide, which reacts with the iron in the medium to form a black precipitate.
BGBB Procedure
Inoculate BGBB with a sample suspected of containing Salmonella or Shigella. Incubate the tubes at 37°C for 24-48 hours.
BGBB Interpretation
- Yellow medium with gas: Salmonella
- Black precipitate: Shigella
- No change: Other bacteria
Essential Questions and Answers on Brilliant Green Bile Broth in "MISCELLANEOUS»UNFILED"
What is Brilliant Green Bile Broth (BGBB)?
Brilliant Green Bile Broth is a selective and differential culture medium used to isolate and identify Salmonella species from food and clinical samples. It contains brilliant green dye, which inhibits the growth of Gram-positive bacteria, and bile salts, which select for Salmonella species.
How does BGBB work?
Brilliant green dye inhibits the growth of Gram-positive bacteria by interfering with their cell membrane function. Bile salts promote the growth of Salmonella species by stimulating the production of bile acids, which are essential for their metabolism.
What are the characteristics of a positive BGBB test?
A positive BGBB test is characterized by the formation of gas and the presence of a yellow-green fluorescence under ultraviolet light. This indicates the presence of Salmonella species.
When is a BGBB test used?
A BGBB test is used to detect the presence of Salmonella species in a variety of samples, including food, water, and clinical specimens. It is a commonly used test for the diagnosis of Salmonella infections.
How reliable is a BGBB test?
BGBB is a highly reliable test for the detection of Salmonella species. It has a high sensitivity and specificity, making it a valuable tool for diagnostic purposes. However, it is important to note that false-positive and false-negative results can occur.
Final Words: BGBB is a valuable diagnostic tool for the isolation and identification of Salmonella and Shigella, which are common causes of gastrointestinal infections. Its selective and differential properties help differentiate between these pathogens and other bacteria, aiding in the accurate diagnosis and appropriate treatment of infections.