What does APBS mean in UNCLASSIFIED
APBS stands for Amphibian Phosphate Buffered Saline. It is a specialized solution used in scientific research to maintain the physiological conditions of amphibian cells and tissues. APBS plays a crucial role in preserving cell viability and functionality during experimental procedures.
APBS meaning in Unclassified in Miscellaneous
APBS mostly used in an acronym Unclassified in Category Miscellaneous that means Amphibian Phosphate Buffered Saline
Shorthand: APBS,
Full Form: Amphibian Phosphate Buffered Saline
For more information of "Amphibian Phosphate Buffered Saline", see the section below.
Composition and Properties
APBS is a modified version of phosphate-buffered saline (PBS), a commonly used buffer solution in biological research. It contains sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate monobasic (NaH2PO4), and sodium phosphate dibasic (Na2HPO4). The specific concentrations of these components are adjusted to mimic the ionic composition of amphibian body fluids, ensuring optimal conditions for cell survival and function.
Applications
APBS is extensively used in a wide range of amphibian research applications, including:
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Cell culture: APBS is employed as a medium for culturing amphibian cells in vitro. It provides the necessary nutrients and ionic balance to maintain cell viability and growth.
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Tissue preservation: APBS is used to preserve amphibian tissues for histological analysis. It prevents cell damage and dehydration, allowing for accurate tissue examination.
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Electrophysiological recordings: APBS is used to create a stable ionic environment for electrophysiological recordings from amphibian neurons and muscle cells.
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Embryo and larval development: APBS is utilized to maintain the proper ionic balance and pH levels during the development of amphibian embryos and larvae.
Advantages of APBS
- Provides optimal ionic conditions for amphibian cells and tissues
- Preserves cell viability and functionality during experimental procedures
- Facilitates embryo and larval development
- Maintains pH levels and ionic balance for electrophysiological recordings
Essential Questions and Answers on Amphibian Phosphate Buffered Saline in "MISCELLANEOUS»UNFILED"
What is APBS (Amphibian Phosphate Buffered Saline)?
APBS is a balanced salt solution specifically designed for use in amphibian research. It mimics the ionic composition of amphibian extracellular fluids, providing an optimal environment for maintaining the health and viability of amphibian cells and tissues.
Why is APBS important for amphibian research?
APBS is essential for a variety of amphibian research applications, including cell culture, tissue transplantation, and electrophysiological studies. Using APBS helps to maintain the proper ionic environment for amphibian cells, ensuring their survival and functionality.
What are the key components of APBS?
APBS typically contains a combination of sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate dibasic (Na2HPO4), and potassium phosphate monobasic (KH2PO4). These components are dissolved in water to create a solution with a specific pH and osmolarity.
How is APBS prepared?
APBS can be prepared by dissolving the appropriate amounts of NaCl, KCl, Na2HPO4, and KH2PO4 in distilled water. The solution is then adjusted to the desired pH and osmolarity using a pH meter and osmometer, respectively.
What are the typical applications of APBS?
APBS is commonly used for:
- Cell culture: Maintaining amphibian cells in vitro
- Tissue transplantation: Preserving amphibian tissue grafts during transplantation procedures
- Electrophysiological studies: Studying the electrical properties of amphibian cells and tissues
- Embryo manipulation: Facilitating the development and manipulation of amphibian embryos
Final Words: APBS is an essential solution in amphibian research, providing a physiologically relevant environment for cell culture, tissue preservation, and various experimental applications. Its unique composition mimics the ionic composition of amphibian body fluids, ensuring optimal conditions for maintaining cell health and functionality throughout the course of scientific investigations.
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