What does CTB mean in UNCLASSIFIED
CTB stands for CellTiter-Blue, a cell viability assay used to quantify the metabolic activity of cells. It is a colorimetric assay that measures the reduction of resazurin to resorufin by live cells. Resazurin is a non-fluorescent blue dye that is reduced to the fluorescent pink dye resorufin by cellular enzymes. The amount of resorufin produced is directly proportional to the number of viable cells present, allowing for the determination of cell viability and proliferation.
CTB meaning in Unclassified in Miscellaneous
CTB mostly used in an acronym Unclassified in Category Miscellaneous that means Cell Titer Blue
Shorthand: CTB,
Full Form: Cell Titer Blue
For more information of "Cell Titer Blue", see the section below.
Applications of CTB Assay
- Cell viability and proliferation studies
- Cytotoxicity assays
- Drug screening
- Monitoring cell culture growth
- Apoptosis and necrosis assays
Advantages of CTB Assay
- Rapid and convenient: The assay is simple and can be performed in a 96-well plate format, making it high-throughput and suitable for large-scale studies.
- Sensitive and quantitative: The assay is highly sensitive and can detect even small changes in cell viability.
- Non-invasive: The assay does not require cell lysis or labeling, allowing for real-time monitoring of cell viability.
- Versatile: The assay can be used with various cell types and culture conditions.
Procedure
- Add CTB solution to cell culture medium.
- Incubate the cells for 1-4 hours.
- Measure the fluorescence or absorbance of the solution.
Essential Questions and Answers on Cell Titer Blue in "MISCELLANEOUS»UNFILED"
What is Cell Titer Blue (CTB)?
Cell Titer Blue is a fluorescent dye used to measure cell viability and proliferation. It is based on the reduction of resazurin to resorufin, which is a highly fluorescent molecule. This reduction is carried out by active mitochondria, which are present in viable cells. Therefore, the fluorescence intensity of CTB is directly proportional to the number of viable cells in a sample.
How is CTB used to measure cell viability?
CTB is added to a cell culture and incubated for a period of time. During this incubation, the resazurin in the CTB is reduced to resorufin by viable cells. The fluorescence intensity of the resulting resorufin solution is then measured using a fluorescence plate reader. The fluorescence intensity is directly proportional to the number of viable cells in the culture.
How is CTB used to measure cell proliferation?
CTB can also be used to measure cell proliferation. In this case, CTB is added to a cell culture and incubated for a period of time. The fluorescence intensity of the resulting resorufin solution is then measured at regular intervals. The increase in fluorescence intensity over time is proportional to the rate of cell proliferation.
What are the advantages of using CTB?
CTB has several advantages over other methods for measuring cell viability and proliferation. These advantages include:
- High sensitivity
- Low cytotoxicity
- Real-time measurement
- Compatibility with a variety of cell types
Final Words: The CTB assay is a powerful tool for assessing cell viability and proliferation. Its simplicity, sensitivity, and versatility make it a valuable technique in various biological research fields, including drug discovery, toxicology, and cell biology.
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