What does PLIC mean in PHOTOGRAPHY & IMAGING
PLIC (Proximity Ligation Imaging Cytometry) is a cutting-edge technique in microscopy used in biological research. It allows researchers to visualize and quantify protein interactions and modifications within intact cells. PLIC combines specific antibody-based targeting with quantitative imaging and analysis, providing unique insights into cellular processes.
PLIC meaning in Photography & Imaging in Miscellaneous
PLIC mostly used in an acronym Photography & Imaging in Category Miscellaneous that means Proximity Ligation Imaging Cytometry
Shorthand: PLIC,
Full Form: Proximity Ligation Imaging Cytometry
For more information of "Proximity Ligation Imaging Cytometry", see the section below.
How does PLIC work?
PLIC employs two primary antibodies, each targeting a specific protein or epitope. These antibodies are conjugated with DNA oligonucleotides complementary to a third oligonucleotide, known as the "linker." When the two antibodies bind to their respective targets in close proximity (typically within 40nm), the linkers hybridize, bringing the two antibodies together. The ligase enzyme then covalently joins these linkers, creating a stable DNA complex.
Applications of PLIC
- Protein-protein interactions: PLIC enables the detection and quantification of protein interactions in their native cellular context. It can reveal the formation of complexes, signaling pathways, and changes in protein interactions under various experimental conditions.
- Protein modifications: PLIC can detect post-translational modifications, such as phosphorylation or ubiquitination, by using antibodies specific to these modifications. It provides information about the subcellular localization and dynamics of modified proteins.
- Disease research: PLIC has applications in studying the molecular mechanisms of diseases by investigating protein interactions and modifications associated with disease states. It can identify potential biomarkers and therapeutic targets.
Essential Questions and Answers on Proximity Ligation Imaging Cytometry in "MISCELLANEOUS»PHOTOGRAPHY"
What is Proximity Ligation Imaging Cytometry (PLIC)?
PLIC is a high-resolution microscopy technique used to investigate protein-protein interactions within the cellular environment. It involves the use of proximity probes to detect and quantify the proximity of specific proteins of interest within a sample.
How does PLIC work?
PLIC utilizes a pair of antibodies conjugated to DNA oligonucleotides. These antibodies target the proteins of interest and, when bound to their respective targets in close proximity, the DNA oligonucleotides are brought together and ligated by a ligase enzyme. The ligated DNA serves as a template for rolling circle amplification, generating a large number of DNA concatemers. The concatemers are then labeled with fluorescent probes for visualization and quantification using microscopy.
What are the advantages of using PLIC?
PLIC offers several advantages, including:
- High specificity: The two-step labeling process with distinct antibodies and DNA probes allows for specific detection of protein interactions, minimizing false positives.
- Quantitative analysis: The number of fluorescent signals corresponds to the proximity and abundance of protein interactions, enabling quantification of protein binding events.
- Spatial resolution: PLIC provides subcellular localization of protein interactions, allowing researchers to investigate the topology and dynamics of protein complexes within the cellular context.
- Applicability to various samples: PLIC can be applied to different sample types, including live cells, fixed cells, and tissue sections.
What types of applications can PLIC be used for?
PLIC has a wide range of applications in biological research, including:
- Investigating protein-protein interactions in signal transduction pathways.
- Studying the assembly and dynamics of protein complexes.
- Identifying novel protein interactions and complexes.
- Assessing the effects of mutations or modifications on protein interactions.
- Quantifying protein interactions in response to cellular stimuli or drug treatments.
What is the sensitivity limit of PLIC?
The sensitivity of PLIC depends on various factors, including the affinity and specificity of the antibodies used and the labeling efficiency. In general, PLIC can detect interactions between proteins that are within a distance of approximately 40 nanometers.
Final Words: PLIC is a powerful technique that has revolutionized the study of protein interactions and modifications in cells. It provides spatial and quantitative information, allowing researchers to gain a deeper understanding of cellular processes. PLIC has broad applications in basic research, drug discovery, and disease diagnostics, contributing to advancements in various biomedical fields.